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Bulk anti-CD62E In Vivo Antibody – Low Endotoxin (CL2)
Product Benefits:
ichorbio’s anti-CD62E In Vivo Antibody – Low Endotoxin (CL2) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
CD62E
Clone:
CL2
Isotype:
Mouse IgG2a
Other Names:
E-selectin, SELE, Endothelial leukocyte adhesion molecule 1, ELAM-1, ELAM1, Leukocyte-endothelial cell adhesion molecule 2, LECAM2
Uniprot:
P16581
Host:
Mouse
Species Reactivity:
Human
Specificity:
Anti-CD62E In Vivo Antibody – Low Endotoxin (CL2) recognizes an epitope on Human CD62E
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
CD62E, also known as E-Selectin, is a type I membrane glycoprotein that belongs to the selectin family of surface molecules which is activated by cytokines and mediates the binding of leukocytes. Like other selectins, it plays an important part in inflammation. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury.
Concentration:
1.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
<1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
Storage:
anti-CD62E In Vivo Antibody – Low Endotoxin (CL2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:
ELISA, Flow Cytometry, Functional Assays, IHC (Frozen), Immunofluorescence
Application Notes:
Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Isotype Control:
Mouse IgG2a In Vivo Isotype Control – Low Endotoxin [C1.18.4] (ICH2245)
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