ichorbio/Anti-CD127 In Vivo Antibody – Low Endotoxin (A7R34) [ICH1071]/1mg/ICH1071,ichorbio,,ichorbio,ichor,+,,Array.Array.Array蚂蚁淘厂家直采.

Anti-CD127 In Vivo Antibody – Low Endotoxin (A7R34) [ICH1071]

$184.00 – $313.00

SKU: ICH1071Categories: anti-mouse, Blocking, Flow Cytometry, Functional Assays, IHC (Frozen), Immunofluorescence, Immunoprecipitation, Low Endotoxin, Western Blot

Bulk anti-CD127 In Vivo Antibody – Low Endotoxin (A7R34)

Product Benefits:

ichorbio’s anti-CD127 In Vivo Antibody – Low Endotoxin (A7R34) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.

Target:

CD127

Clone:

A7R34

Isotype:

Rat IgG2a

Other Names:

Interleukin-7 receptor subunit alpha, Il7r, IL-7R subunit alpha, IL-7R-alpha, IL-7RA

Uniprot:

P16872

Host:

Rat

Species Reactivity:

Mouse

Specificity:

Anti-CD127 In Vivo Antibody – Low Endotoxin (A7R34) recognizes an epitope on Mouse CD127

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:

IL-7 receptor alpha (IL-7Ra), also known as CD127, is expressed on bone marrow lymphoid precursors, pro-B cells, monocytes/macrophages, natural killer (NK) cells, naïve T cells and memory CD4+ and CD8+ T cells. IL-7Ra is also expressed on non-immune somatic cells such as intestinal colorectal and renal carcinoma cell lines and keratinocytes. IL-7Ralpha displays a synergistic decrease in the number of T-cell, NK-cell and multipotent progenitors in the thymus, indicating that these distinct survival pathways converge to promote the development of multipotent lymphoid progenitors.

Immunogen:

IL-7Ra-IgG1 fusion protein

Concentration:

1.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Storage:

anti-CD127 In Vivo Antibody – Low Endotoxin (A7R34) is stable when stored sterile at 2-8°C. Do not freeze.

Applications:

Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry, IHC (Frozen), Immunofluorescence

Application Notes:

Blocking: Clone A7R34 is able to block clone SB/199 binding to IL-7R.Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Isotype Control:

Rat IgG2a In Vivo Isotype Control – Low Endotoxin [1-1] (ICH2244)