ichorbio/Anti-CD278 In Vivo Antibody – Low Endotoxin (7E.17G9) [ICH1082]/100mg/ICH1082,ichorbio,,ichorbio,ichor,+,,Array.Array.Array蚂蚁淘厂家直采.

Anti-CD278 In Vivo Antibody – Low Endotoxin (7E.17G9) [ICH1082]

$320.00 – $2,378.00

SKU: ICH1082Categories: anti-mouse, Blocking, Flow Cytometry, Functional Assays, Low Endotoxin, Ultra Low Endotoxin, Western Blot

Bulk anti-CD278 In Vivo Antibody – Low Endotoxin (7E.17G9)

Product Benefits:

ichorbio’s anti-CD278 In Vivo Antibody – Low Endotoxin (7E.17G9) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.

Target:

CD278

Clone:

7E.17G9

Isotype:

Rat IgG2b

Other Names:

Inducible T-cell costimulator, Icos, Activation-inducible lymphocyte immunomediatory molecule, CD28 and CTLA-4-like protein, CCLP, CD28-related protein 1, CRP-1, Ailim

Uniprot:

Q9WVS0

Host:

Rat

Species Reactivity:

Mouse

Specificity:

Anti-CD278 In Vivo Antibody – Low Endotoxin (7E.17G9) recognizes an epitope on Mouse ICOS

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:

Inducible co-stimulator (ICOS), also called ALIM and H4, is a homodimeric protein and member of the CD28 family of immune co-stimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. ICOS enhances all basic T cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B cells. Unlike the constitutively expressed CD28, ICOS has to be de novo induced on the T cell surface, does not up-regulate the production of IL-2, but superinduces the synthesis of IL-10, a B cell-differentiation factor. In vivo, ICOS is highly expressed on tonsillar T cells, which are closely associated with B cells in the apical light zone of germinal centres, the site of terminal B cell maturation. ICOS regulates CD28-dependent and CD28-independent CD4 (+) subset (Th1 and Th2) responses. It is a major regulator of the adaptive immune system and therefore, has a protective role in inflammatory autoimmune diseases.

Concentration:

1.0 – 5.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:Mycoplasma sppMycoplasma pulmonisPneumonia virus of miceKilham’s rat virusToolan’s H1 virusRat parvovirusLymphocytic choriomeningitis virusRat cytomegalovirusSendai virusRat coronavirusSialodacryoadenitis virusRat minute virusSeoul virusMouse adenovirusReovirus 3Rat theilovirus

Storage:

anti-CD278 In Vivo Antibody – Low Endotoxin (7E.17G9) is stable when stored at 2-8°C. Do not freeze. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Applications:

Western Blot, Blocking, Functional Assays, Flow Cytometry

Application Notes:

Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Isotype Control:

Rat IgG2b In Vivo Isotype Control – Low Endotoxin [1-2] (ICH2243)

Related Antibody:

Anti-CD278 In Vivo Antibody – Ultra Low Endotoxin [7E.17G9] (ICH1082UL)