ichorbio/Anti-CD45R In Vivo Antibody – Low Endotoxin (RA3-6B2) [ICH1130]/100mg/ICH1130,ichorbio,,ichorbio,ichor,+,,Array.Array.Array蚂蚁淘厂家直采.

Anti-CD45R In Vivo Antibody – Low Endotoxin (RA3-6B2) [ICH1130]

$320.00 – $2,378.00

SKU: ICH1130Categories: anti-human, anti-mouse, ELISA, Flow Cytometry, Functional Assays, IHC (Frozen), IHC (Paraffin), Immunofluorescence, Immunoprecipitation, Low Endotoxin, Ultra Low Endotoxin, Western Blot

Bulk anti-CD45R In Vivo Antibody – Low Endotoxin (RA3-6B2)

Product Benefits:

ichorbio’s anti-CD45R In Vivo Antibody – Low Endotoxin (RA3-6B2) is manufactured in a cGMP compliant facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.

Target:

CD45R

Clone:

RA3-6B2

Isotype:

Rat IgG2a

Other Names:

Leukocyte common antigen, L-CA, T200, CD45, Receptor-type tyrosine-protein phosphatase C, PTPRC, B220

Uniprot:

P08575

Host:

Rat

Species Reactivity:

Mouse, Human

Specificity:

Anti-CD45R In Vivo Antibody – Low Endotoxin (RA3-6B2) recognizes the B-cell determinant (Mr 220 kD) of the CD45 molecule.

Antigen Distribution:

The CD45R antigen is present on mouse B-cells, B-cell precursors, T cell subset, NK cell subsets .

Background:

CD45R, also known as B220 is a 220 kDa transmembrane protein tyrosine phosphatase expressed on B cells and some subsets of T and NK cells. This antibody is commonly used as a B cell marker as CD45R does play a critical role in TCR and BCR signaling.

Immunogen:

Abelson murine leukemia virus-induced pre-B tumor cells

Concentration:

0.5 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:Mycoplasma sppMycoplasma pulmonisPneumonia virus of miceKilham’s rat virusToolan’s H1 virusRat parvovirusLymphocytic choriomeningitis virusRat cytomegalovirusSendai virusRat coronavirusSialodacryoadenitis virusRat minute virusSeoul virusMouse adenovirusReovirus 3Rat theilovirus

Storage:

Anti-CD45R In Vivo Antibody – Low Endotoxin (RA3-6B2) is stable for at least four (4) weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –80°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Applications:

Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), IHC (Paraffin), Immunoprecipitation, Immunofluorescence, ELISA

Application Notes:

Functional Assay: In vivo treatment with anti-CD45R (Clone RA3-6B2) reduces the number of T and B cells and modifies their functional activity.Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Isotype Control:

Rat IgG2a In Vivo Isotype Control – Low Endotoxin [1-1] (ICH2244)

Related Antibody:

Anti-CD45R In Vivo Antibody – Ultra Low Endotoxin [RA3-6B2] (ICH1130UL)

Antibodies against the same target:

Anti-CD45 In Vivo Antibody – Low Endotoxin [I3/2.3] (ICH1057)