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Anti-CD4 In Vivo Antibody – Low Endotoxin (GK1.5) [ICH1042]

$320.00 – $2,378.00

SKU: ICH1042Categories: anti-mouse, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation, Low Endotoxin, Ultra Low Endotoxin, Western Blot

Bulk anti-CD4 In Vivo Antibody – Low Endotoxin (GK1.5)

Bio X Cell:

ICH1042 is up to 19% cheaper for academia & non-profits and up to 52% cheaper for industry than the equivalent products BE0003-1 (low endotoxin) and BP0003-1 (ultra-low endotoxin) from Bio X Cell.We guarantee that our antibody will perform exactly the same in your experiments as BE/BP0003-1 from Bio X Cell: if it doesn’t we will refund your purchase and pay for the Bio X Cell antibody to be delivered to you as soon as possible.

Product Benefits:

ichorbio’s anti-CD4 In Vivo Antibody – Low Endotoxin (GK1.5) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.

Target:

CD4

Clone:

GK1.5

Size:

ichorbio’s GK1.5 in vivo antibody is available in the following bulk sizes:1mg, 5mg, 25mg, 50mg and 100mgichorbio regularly manufactures multi-gram amounts of our anti-CD4 GK1.5 clone – please contact us for pricing.

Isotype:

Rat IgG2b

Other Names:

T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3

Host:

Rat

Species Reactivity:

Mouse

Specificity:

Anti-CD4 In Vivo Antibody – Low Endotoxin (GK1.5) recognizes an epitope on Mouse CD4

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.

Background:

CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.

Immunogen:

Mouse CTL clone V4

Concentration:

≥ 2.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:Mycoplasma sppMycoplasma pulmonisPneumonia virus of miceKilham’s rat virusToolan’s H1 virusRat parvovirusLymphocytic choriomeningitis virusRat cytomegalovirusSendai virusRat coronavirusSialodacryoadenitis virusRat minute virusSeoul virusMouse adenovirusReovirus 3Rat theilovirus

Storage:

anti-CD4 In Vivo Antibody – Low Endotoxin (GK1.5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Applications:

Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation

Application Notes:

Blocking: Anti-Mouse CD4 (clone GK1.5) is useful for In vitro and In vivo studies of T-helper cell functions including blocking of ligand binding and T-helper cell activation.Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Isotype Control:

Rat IgG2b In Vivo Isotype Control – Low Endotoxin [1-2] (ICH2243)

Related Antibody:

Anti-CD4 In Vivo Antibody – Ultra Low Endotoxin [GK1.5] (ICH1042UL)

Antibodies against the same target:

Anti-CD4 In Vivo Antibody – Low Endotoxin [RPA-T4] (ICH1004), Anti-CD4 In Vivo Antibody – Ultra Low Endotoxin [RPA-T4] (ICH1004UL)