Bulk anti-CD151 In Vivo Antibody – Low Endotoxin (50-6)
Product Benefits:
ichorbio’s anti-CD151 In Vivo Antibody – Low Endotoxin (50-6) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
CD151
Clone:
50-6
Isotype:
Mouse IgG1
Other Names:
GP27, Membrane glycoprotein SFA-1, Platelet-endothelial tetraspan antigen 3, PETA-3, Tetraspanin-24, Tspan-24
Uniprot:
P48509
Host:
Mouse
Species Reactivity:
Human
Specificity:
Anti-CD151 In Vivo Antibody – Low Endotoxin (50-6) recognizes an epitope on Human CD151
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
CD151 is found on Endothelium, muscle, epithelial cells, platelets, and megakaryocytes, and immature hemopoietic cells. Also found on Activated T-cells.
Background:
The clone 50-6 has been shown to inhibit tumor cell metastasis in human carcinoma cell lines.
Concentration:
1.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
<0.5 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
Storage:
anti-CD151 In Vivo Antibody – Low Endotoxin (50-6) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:
Western Blot, Blocking
Application Notes:
Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Isotype Control:
Mouse IgG1 Isotype Control for In Vivo – Low Endotoxin [HKSP] (ICH2247)
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